Module

Biosafety in Biology and Chemistry

Introducing the chemical hood, the biosafety cabinet, the autoclave,  different regimes of autoclaving, and biosafety lab levels

Module 1.0	Laboratory-based environmental toxicity (bioassay) testing using Fast Plant seeds
	1.1 Activities:	Bioassay experimental design, hypotheses testing, preparing extractions, dilutions, microscopy, data analysis
Module 2.0	Field-based assessment of physical and chemical surface water quality indicators associated with an urban stream
	2.1 Activities:	Sampling protocols, calibration of pH, temperature, and TDS field measurement instrumentation, colorimeter analysis for dissolved oxygen and orthophosphate, data analysis
Module 3.0	Field/laboratory characterization of biological indicators associated with an urban stream (chlorophyll a, macroinvertebrates, bacteria)
	3.1 Activities:	Sampling protocols, dichotomous keys, diversity index determination, preparing extractions, spectrophotometric analysis, enumeration of bacterial populations (plating/sterile technique), data analysis

Module 4.0	The relationship between an enzyme and its substrate molecule(s)
	4.1 Activities:	Inositol trisphosphate functions in cells
Module 5.0	Relate the function of the plasma (cell)  membrane to its structure
	5.1 Activities:	Introduction of cell membranes of various cells, role of inositol   trisphosphate
Module 6.0	Compare and contrast active transport and passive transport mechanisms:   • diffusion   • osmosis   • endocytosis   • exocytosis   • phagocytosis   • pinocytosis
	6.1 Activities:	Sampling protocols, preparing extractions, spectrophotometric analysis, enumeration of cells (plating/sterile technique), data analysis

Module 7.0	Carbohydrates, disaccharides, and polysaccharides
	7.1 Activities:	Sampling protocols, preparing serial dilutions, spectrophotometric analysis, data analysis
Module 8.0	Prokaryotic and eukaryotic cells, and role carbohydrates, disaccharides, and polysaccharides
	8.1 Activities:	Recognition assay using carbohydrate and carbohydrate polymers, experimental design, hypotheses testing, preparing extractions, dilutions, data analysis
Module 9.0	Compare and carbohydrate structure and their role on biological function:    • binding    • recognition    • phagocytosis
	9.1 Activities:	Sampling protocols, spectrophotometric analysis, enumeration of bacterial populations (plating/sterile technique), data analysis

June-August, 2012	Preparation / submission of abstracts on the obtained results to the 6th BioNanoTox and  Applications Research Conference
August-September	Printing posters based on prepared PowerPoint presentations
November 15-16, 2012	Participations and poster presentations at the 6th BioNanoTox and Applications Research  Conference,  November 15-16, 2012
November-December, 2012	Optional: Preparation / submission of articles on the obtained results to be published Articles  will be published by the BioNanoTox International Journal in April-May, 2013.